Stool specimen collection in Pediatrics

Pediatric Nursing

Stool specimen collection in pediatrics is the process of obtaining a child’s fecal sample for diagnostic testing. It helps detect infections, parasites, blood, fat malabsorption, or digestive disorders. The method varies by age and clinical need, with a strong emphasis on hygiene and minimizing contamination.

Common Indications
PurposeExamples
Detect gastrointestinal infectionsBacterial, viral, or parasitic pathogens
Screen for occult bloodSuspected GI bleeding or anemia
Evaluate digestive functionFat malabsorption, enzyme deficiencies
Monitor chronic conditionsInflammatory bowel disease, food allergies
Preparation for Stool specimen collection
  • Explain the parents and the child (as appropriate) the need for collecting stool specimen.
  • Provide privacy in the case of older children
Procedure

       Put on the gloves

  • For older children:
  • Patients can collect the specimen by passing feces into plastic wrap stretched loosely over the toilet bowl.
  • A portion of the sample is then transferred into the supplied container with the help of a spatula.
  • For younger children and infants:
  • The diaper should be lined with plastic wrap.
  • A urine bag can be attached to the child to ensure that stool specimen is not contaminated with urine.
Aftercare
  • The child should be made clean and comfortable.
  • All contents of kit, towels, plastic wrap, and gloves should be disposed of in appropriate containers.
  • Wash your hands thoroughly.
  • Speed in testing the sample is essential, in order to obtain the accurate result.
    Therefore, the specimen should be sent for testing as quickly as possible.
  • Document the procedure: Time of specimen collection, stool color, amount, consistency and odor, the test to be performed, and the skin condition.
General Instructions
  • For testing ova and parasites, specimen collection containers that contain chemical preservatives must be used.
  • For culture, the best samples are of loose, fresh stool, sometimes, more than one stool will be collected for a culture.
  • Swabs from a child’s rectum can also be tested for viruses (occasionally).
  • Testing for blood in the stool is often performed with a quick test. First stool is smeared on a card, and then few drops of a developing solution are placed on the card. An instant color shows that blood is present in the stool
Tips for Caregivers
  • Reassure the child: Let them know it’s painless and important for their health.
  • Use child-friendly language: Say “we need a poop sample to help the doctor understand your tummy.”
  • For infants: Line the diaper with plastic wrap to catch stool without urine contamination.
  • For older children: Use a toilet hat or stretch plastic wrap under the seat to catch stool before it touches water.
Special Considerations
  • For ova and parasite testing, use containers with preservatives.
  • For occult blood, avoid red meat and certain medications before collection.
  • Document time, consistency, color, and any unusual findings.

REFERENCES

  1. Annamma Jacob, Rekha, Jhadav Sonali Tarachand: Clinical Nursing Procedures: The Art of Nursing Practice, 5th Edition, March 2023, Jaypee Publishers, ISBN-13: 978-9356961845 ISBN-10: 9356961840
  2. Omayalachi CON, Manual of Nursing Procedures and Practice, Vol 1, 3 Edition 2023, Published by Wolters Kluwer’s, ISBN: 978-9393553294
  3. Sandra Nettina, Lippincott Manual of Nursing Practice, 11th Edition, January 2019, Published by Wolters Kluwer’s, ISBN-13:978-9388313285
  4. Marcia London, Ruth Bindler, Principles of Paediatric Nursing: Caring for Children, 8th Edition, 2023, Pearson Publications, ISBN-13: 9780136859840.
  5. Ernstmeyer K, Christman E, editors. Nursing Fundamentals [Internet]. 2nd edition. Eau Claire (WI): Chippewa Valley Technical College; 2024. PART IV, NURSING PROCESS. Available from: https://www.ncbi.nlm.nih.gov/books/NBK610818/

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